![]() ![]() Our research will provide new clues for understanding biological characteristics, development and function of immune cell populations of teleost. mature B cells, immature B cells, pre B cells and early-pre B cells. Further analysis indicated the presence of two subsets of Mo/MΦ including M1 and M2 type, as well as four subsets in B cells, i.e. Based on cell heterogeneity and known markers, we characterized four cell types: T cells, B cells, monocytes/macrophages (Mo/MΦ) and NCC (non-specific cytotoxic cells). We show that it reconstructs well most of the present transcripts in the scRNA-seq data achieving a sensitivity equivalent to approaches relying on genome alignments of related species. scRNA-seq data analysis with a full-length transcriptome as a reference, and evaluate this approach on samples from Epinephelus coioides-a teleost without any published genome. ![]() Current approaches for scRNA-seq data analysis usually rely on comparison with a reference genome and hence are not suited for samples without any reference genome, which is currently very common in fish research. Single cell RNA-seq (scRNA-seq) has provided a new approach for effective in-depth identification and characterization of cell subpopulations. However, the knowledge of distinct immune cell populations in fish is still limited, and further development of techniques advancing the identification of fish immune cell populations and their functions are required. 5Institute of Biochemistry and Biology, Postdam University, Potsdam, Germanyįish is considered as a supreme model for clarifying the evolution and regulatory mechanism of vertebrate immunity.4Department of Experimental Limnology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Stechlin, Germany. ![]() 3Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.2Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai, China.1Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, China.Lixing Huang 1† Ying Qiao 2† Wei Xu 3 Linfeng Gong 3 Rongchao He 1 Weilu Qi 1 Qiancheng Gao 1 Hongyan Cai 1 Hans-Peter Grossart 4,5* Qingpi Yan 1* ![]()
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